Determination of Ro 15-1788, a Benzodiazepine Antagonist, in Human Plasma by Gas-Liquid Chromatography with Nitrogen-Phosphorus Detection

Autor:	Lauven Pm, Rainer M. Arendt, Darrell R. Abernethy, David J. Greenblatt
Rok vydání:	1983
Předmět:	Pharmacology Chromatography Extraction (chemistry) Antagonist Ethyl acetate General Medicine Standard curve chemistry.chemical_compound Pharmacokinetics chemistry Flumazenil medicine Sample preparation Gas chromatography medicine.drug
Zdroj:	Pharmacology. 26:285-289
ISSN:	1423-0313 0031-7012
DOI:	10.1159/000137812
Popis:	A sensitive (to 3 ng/ml) and specific method for analysis of the benzodiazepine antagonist Ro 15-1788 is described. The method utilizes gas-liquid chromatography with nitrogen-phosphorus detection. A neutral pH ethyl acetate extraction of 0.1-3.0 ml plasma is used for sample preparation. Standard curves using methylclonazepam as the internal standard are linear for plasma concentrations up to 200 ng/ml. Applicability of the method is demonstrated by a pharmacokinetic study in a normal volunteer who received 10 mg Ro 15-1788 intravenously.
Databáze:	OpenAIRE
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