Effect of rhythmic auxin application on shoot morphogenesis in carrot (Daucus carotaL.) callus tissue
| Autor: | Vali Borimnejad, Ebrahim Hadavi, Noushin Ghazijahani, H. Fahimi, Ahmad Majd |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Physiology fungi food and beverages Organogenesis Biology biology.organism_classification Plant development chemistry Distilled water Auxin Physiology (medical) Callus Shoot Botany heterocyclic compounds Shoot morphogenesis Ecology Evolution Behavior and Systematics Daucus carota |
| Zdroj: | Biological Rhythm Research. 43:671-680 |
| ISSN: | 1744-4179 0929-1016 |
| DOI: | 10.1080/09291016.2011.631791 |
| Popis: | The possible role of auxin oscillations in plant development has been considered earlier but direct experimental evidence in support of this concept has been limited. In this research, we conducted a series of experiments by employing an instrument of novel design that dispensed auxin droplets on the surface of callus cultures in fixed amounts at controllable time intervals (frequencies). The effects of 60 h exposures of different indole-acetic acid (IAA) concentrations (0.5, 1 and 2 mg l−1) and dispensing time intervals (6, 9, 12, 13.5, 15, 18 and 21 min) were tested on carrot calli and compared with control treatments with steady exposure to IAA and rhythmic application of distilled water. Shoot organogenesis was observed after two weeks only on calli that were exposed to IAA oscillations of 9 and 18 min, while other time intervals and controls, including non-periodical controls and periodical application of distilled water, were ineffective. The results were similar among different IAA concentrations. ... |
| Databáze: | OpenAIRE |
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