Use of a gaseous electrical discharge to induce fluorescence in organic compounds separated by thin-layer chromatography

Autor: F. Hsu, H. Shanfield, A.J.P. Martin
Rok vydání: 1976
Předmět:
Zdroj: Journal of Chromatography A. 126:457-462
ISSN: 0021-9673
DOI: 10.1016/s0021-9673(01)84092-9
Popis: It has been found that a simple gaseous electrical discharge can induce visible fluorescence in a variety of organic compounds separated on silica gel thin-layer chromatoplates. Exposure times ranging from 5 sec to 5 min are ample to detect submicrogram quantities with the unaided eye. The method is simple and convenient, and appears to have broad applicability to a wide variety of organic compounds. It is anticipated that this procedure will lend itself to sensitive quantitation by the use of spectrofluorimetric instrumentation.
Databáze: OpenAIRE