| Autor: |
Fredrik Hurtig, Thomas C. Q. Burgers, Alice Cezanne, Xiuyun Jiang, Frank N. Mol, Jovan Traparić, Andre Arashiro Pulschen, Tim Nierhaus, Gabriel Tarrason-Risa, Lena Harker-Kirschneck, Jan Löwe, Anđela Šarić, Rifka Vlijm, Buzz Baum |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Science Advances. 9 |
| ISSN: |
2375-2548 |
| Popis: |
ESCRT-III family proteins form composite polymers that deform and cut membrane tubes in the context of a wide range of cell biological processes across the tree of life. In reconstituted systems, sequential changes in the composition of ESCRT-III polymers induced by the AAA–adenosine triphosphatase Vps4 have been shown to remodel membranes. However, it is not known how composite ESCRT-III polymers are organized and remodeled in space and time in a cellular context. Taking advantage of the relative simplicity of the ESCRT-III–dependent division system in Sulfolobus acidocaldarius , one of the closest experimentally tractable prokaryotic relatives of eukaryotes, we use super-resolution microscopy, electron microscopy, and computational modeling to show how CdvB/CdvB1/CdvB2 proteins form a precisely patterned composite ESCRT-III division ring, which undergoes stepwise Vps4-dependent disassembly and contracts to cut cells into two. These observations lead us to suggest sequential changes in a patterned composite polymer as a general mechanism of ESCRT-III–dependent membrane remodeling. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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