A novel method to measure the 13C composition of intact bacteriohopanepolyols
| Autor: | Catherine Ann Polik, Felix J Elling, Sunita R. Shah Walter, Stephanie Kusch, Jordon D. Hemingway, Ann Pearson |
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| Rok vydání: | 2018 |
| Předmět: |
Bacteriohopanetetrol
Chromatography 010504 meteorology & atmospheric sciences Isotope Geochemistry and Petrology Chemistry Composition (visual arts) 010502 geochemistry & geophysics Mass spectrometry 01 natural sciences High-performance liquid chromatography 0105 earth and related environmental sciences |
| Zdroj: | Organic Geochemistry. 123:144-147 |
| ISSN: | 0146-6380 |
| DOI: | 10.1016/j.orggeochem.2018.07.002 |
| Popis: | We present a novel method to measure the 13C/12C isotope ratio (reported as δ13C) of individual, intact bacteriohopanepolyols (BHPs) using semi-preparative ultrahigh pressure liquid chromatography (UPLC) followed by high temperature gas chromatography–isotope ratio mass spectrometry (HT-GC–IRMS). The method is reproducible, as indicated by the precision of δ13C values for bacteriohopanetetrol (BHT) extracted from R. palustris biomass and analyzed across an order-of-magnitude range of IRMS peak areas (δ13C = −33.4 ± 0.6‰ VPDB, n = 94, ±1σ). To show that this method successfully separates individual BHPs from complex environmental matrices, we report δ13C values for BHT and the BHT stereoisomer (BHT-II) from a ca. 2.9 Ma, organic-rich Mediterranean Sea sediment sample. These analyses suggest that intact BHP δ13C measurements can greatly improve the interpretation of environmental signals by alleviating the need for side-chain cleavage which reduces BHP source-specificity. |
| Databáze: | OpenAIRE |
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