Genetic analysis of Streptomyces albus J1074 mia mutants suggests complex relationships between post-transcriptional tRNAXXA modifications and physiological traits
| Autor: | Roderich D. Süssmuth, Oksana Koshla, Bohdan Ostash, Volodymyr Kravets, Iryna Ostash, Yuriy Dacyuk |
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| Rok vydání: | 2020 |
| Předmět: |
Genetics
Aerial mycelium formation 0303 health sciences biology 030306 microbiology Mutant General Medicine biology.organism_classification Microbiology Streptomyces Genetic analysis 03 medical and health sciences Transfer RNA Secondary metabolism Gene Streptomyces albus 030304 developmental biology |
| Zdroj: | Folia Microbiologica. 65:1009-1015 |
| ISSN: | 1874-9356 0015-5632 |
| Popis: | Proteins MiaA and MiaB catalyze sequential isopentenylation and methylthiolation, respectively, of adenosine residue in 37th position of tRNAXXA. The mia mutations were recently shown by us to affect secondary metabolism and morphology of Streptomyces. However, it remained unknown as to whether both or one of the aforementioned modifications is critical for colony development and antibiotic production. Here, we addressed this issue through analysis of Streptomyces albus J1074 strains carrying double miaAmiaB knockout or extra copy of miaB gene. The double mutant differed from wild-type and miaA-minus strains in severity of morphological defects, growth dynamics, and secondary metabolism. Introduction of extra copy of miaB gene into miaA mutant restored aerial mycelium formation to the latter on certain solid media. Hence, miaB gene might be involved in tRNA thiomethylation in the absence of miaA; either MiaA- or MiaB-mediated modification appears to be enough to support normal metabolic and morphological processes in Streptomyces. |
| Databáze: | OpenAIRE |
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