Expression and Regulation of Regulated on Activation, Normal T Cells Expressed and Secreted in Thyroid Tissue of Patients with Graves’ Disease and Thyroid Autonomy and in Thyroid-Derived Cell Populations1
| Autor: | D. Sittig, Irina Lehmann, C. Simchen, Gabriela Aust, M. Steinert |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Chemokine
medicine.medical_specialty endocrine system diseases Endocrinology Diabetes and Metabolism Graves' disease Biochemistry (medical) Clinical Biochemistry Thyroid CCR4 Biology medicine.disease Biochemistry Endocrinology medicine.anatomical_structure Thyroid peroxidase Cell culture Internal medicine Gene expression biology.protein medicine Receptor |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 85:4758-4764 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.85.12.7082 |
| Popis: | Thyroid glands affected by Graves’ disease (GD) show striking lymphocytic infiltration, mainly by CD45RO+ T cells. The mechanisms by which the various lymphocytic subsets are recruited and maintained in the thyroid are unknown. RANTES (regulated on activation, normal T cells expressed and secreted) in interaction with its receptors (CCR1, CCR3, CCR4 and CCR5) may be one of the favorite chemokines involved in the cell trafficking and maintenance. RANTES messenger RNA (mRNA) was quantified in the thyroid tissue of 16 patients with GD and 7 patients with thyroid autonomy (TA), using competitive RT-PCR. We found a clear correlation between the RANTES mRNA level and 1) the degree of T-cell infiltration (r = 0.68), and 2) the level of serum antibodies to thyroid peroxidase (r = 0.76) in GD but not in TA patients. There was no difference between the autonomous nodules and the quiescent surrounding tissue in TA patients. To define the cellular source of RANTES mRNA and protein, we examined various thyroid-derived... |
| Databáze: | OpenAIRE |
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