Identification of sesame phyllody transmitting insect vectors in Assam, India
| Autor: | M. K. Kalita, Shankar Hemanta Gogoi, Sahidur Rahman, Palash Deb Nath, Jutimala Phookan |
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| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Veterinary medicine education.field_of_study biology Inoculation Population Cell Biology biology.organism_classification 16S ribosomal RNA Aster yellows Leafhopper Infectious Diseases Phytoplasma Parasitology Phyllody education Nested polymerase chain reaction Ecology Evolution Behavior and Systematics |
| Zdroj: | Phytopathogenic Mollicutes. 9:107 |
| ISSN: | 2249-4677 2249-4669 |
| DOI: | 10.5958/2249-4677.2019.00054.9 |
| Popis: | Different leafhopper species transmitting sesame phyllody phytoplasma were reported. Leafhoppers were collected from sesame phyllody infected field at different time interval and their population was recorded. Four leafhopper species were predominantly observed in the field conditions: Hishimonus phycitis, Exitianus indicus, Cofana unimaculata and Nephotettix nigropictus. Transmission studies were carried out by giving an acquisition and inoculation feeding period of 7 days each. Among all the leafhoppers, H. phycitis was found to transmit sesame phyllody phytoplasma (83.33%) to healthy sesame plants as confirmed by PCR and nested PCR assays followed by sequencing. The BLAST analysis of 16S rDNA sequences from H. phycitis confirmed the phytoplasma presence indicating that is clusters with the aster yellows phytoplasmas. |
| Databáze: | OpenAIRE |
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