Recycled Paper Sludge Microbial Community as a Potential Source of Cellulase and Xylanase Enzymes
| Autor: | Fernanda J. Trindade, Lorena Benathar Ballod Tavares, Kássia Gisele Hackbarth Heinz, Adriana Giongo, Rafael Rodrigues de Oliveira, P. R. S. Zanoni, Leandro de Mattos Pereira, Renata Medina-Silva, Taiz L. L. Simão |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Environmental Engineering Enhydrobacter Renewable Energy Sustainability and the Environment Firmicutes business.industry 030106 microbiology Cellulase Biology Pulp and paper industry biology.organism_classification Industrial waste Biotechnology 03 medical and health sciences 030104 developmental biology Bioproducts biology.protein Xylanase Cloacibacterium business Waste Management and Disposal Waste disposal |
| Zdroj: | Waste and Biomass Valorization. 8:1907-1917 |
| ISSN: | 1877-265X 1877-2641 |
| Popis: | Waste disposal from paper and pulp industry is of great environmental concern. Sludge from recycled paper mills favors colonization with different microbial species due to its chemical composition. In this environment, the microbiota can express enzymes that hydrolyze celluloses and hemicelluloses, such as cellulases, β-glucosidases, and xylanases. Understanding microbiota living in such disposal may give directions to what kind of enzymes or bioproducts may be possible to obtain from this microorganism and how to deal with the waste during the process. We have accessed microbial community associated with two steps in the recycled paper sludge generation by high-throughput DNA sequencing: fibrous residue, and wastewater sludge. Bacteroidetes, Proteobacteria and Firmicutes phyla represented more than 96% of the total. From the eukaryote perspective, Vorticella was the most frequent in fibrous residue samples while Acanthamoeba was the most common on wastewater sludge samples. Among the isolates cellulase activity was detectable only from fungi isolates. Half of the bacterial isolates presented xylanase activity, and most of them in high level of activity. Both a metabarcoding and culturing approaches are valuable tools for enzymatic screening in industrial waste disposal. |
| Databáze: | OpenAIRE |
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