Ultrastructural and Physiological Characterization of YELP, a Novel, Yellow, Chlorophyll-Deficient Cell Mutant Line That Develops Etioplast-Like Plastids in the Light
| Autor: | Edmundo García-Moya, Betzaida Jiménez-Francisco, Florentino Víctor Conde-Martínez, Carlos Trejo, Hilda Araceli Zavaleta-Mancera, Gerardo Armando Aguado-Santacruz, Blanca Moreno-Gómez |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification Chlorophyll b food and beverages General Medicine Biology Photosynthesis 01 natural sciences Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Etioplast Xanthophyll Chlorophyll Ultrastructure Plastid Accessory pigment 010606 plant biology & botany |
| Zdroj: | American Journal of Plant Sciences. :510-524 |
| ISSN: | 2158-2750 2158-2742 |
| Popis: | In this work, we present an ultrastructural and physiological description of a novel chlorophyll-deficient, yellow cell line of the grass Bouteloua gracilis that develops etioplast-like plastids in presence of light (YELP). These mutant cells were compared to the parental, wild-type, highly chlorophyllous cells from which they were isolated. Growth analysis, based on fresh and dry weights, indicated that YELP accumulates biomass at a slower rate than the parental, green cells. Besides, YELP developed very low levels of photosynthetic pigments, reaching only 9.3% and 38.4% of chlorophyll a and chlorophyll b, respectively, developed by the wild-type cells. Likewise, the accessory pigments, carotenes and xanthophylls, were only synthesized at 8.0% and 5.4%, respectively, of the levels reached by the green cells. Electron microscopy revealed remarkable differences in plastid ultrastructure between the wild-type and mutant cells. Plastids of YELP were heterogeneous and smaller than those found in wild-type cells. YELP plastids were abnormal with poorly developed membrane systems that prevented the accumulation of chlorophyll and accessory pigments in the mutant cell line. We expect this novel, mutant cell line will provide new tools for studying plastid development and differentiation. |
| Databáze: | OpenAIRE |
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