Phytic acid-based copper(ii ) ion-selective electrode on graphene oxide for potentiometric immunoassay of breast cancer antigen 15-3
| Autor: | Xi Shi, Min Zheng, Wei-Hua Cai, Mou-Feng Wang, Yong-Jin Cao, Yu-Jie Deng |
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| Rok vydání: | 2019 |
| Předmět: |
Detection limit
medicine.diagnostic_test Chemistry Potentiometric titration 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Catalysis 0104 chemical sciences Ion selective electrode Linear range Immunoassay Electrode Materials Chemistry medicine Chelation 0210 nano-technology Electrode potential Nuclear chemistry |
| Zdroj: | New Journal of Chemistry. 43:11171-11177 |
| ISSN: | 1369-9261 1144-0546 |
| DOI: | 10.1039/c9nj01834d |
| Popis: | A potentiometric immunoassay based on a copper(II) ion-selective electrode (Cu-ISE) was designed for the quantitative determination of carbohydrate antigen 15-3 (CA 15-3; a tumor marker associated with breast cancer). To construct such a Cu-ISE-based assay system, a sandwiched immunoreaction was carried out on a capture antibody-coated microplate by using a cupric oxide (CuO) nanoparticle-labeled detection antibody, whereas the Cu-ISE was prepared on a gold-disk electrode by using phytic acid-functionalized graphene oxide nanosheets as the transducer. Followed by forming the immunocomplexes, the dissolved Cu2+ ions from CuO nanoparticles by acids were specifically chelated with immobilized phytic acid on the electrode because of the highly efficient complexing ability, thereby resulting in a change of the electrode potential. Under optimum conditions, the Cu-ISE-based potentiometric immunosensor yielded a near Nernstian slope (12.01 ± 0.66 mV dec−1) for the detection of CA 15-3 within a dynamic linear range of 0.01–100 U mL−1 with a low detection limit of 5.3 mU mL−1. Moreover, the Cu-ISE-based immunoassay showed good reproducibility, high specificity and long-time stability. Furthermore, it was applied for the determination of CA 15-3 in human serum specimens, providing well-matched results in comparison with the referenced CA 15-3 ELISA kit. Importantly, the Cu-ISE-based immunoassay provides a promising alternative for simple and reliable screening of disease-related biomarkers. |
| Databáze: | OpenAIRE |
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