Hypoxia Induces Intestinal Isocitrate Dehydrogenase Expression and Enhances 13 C-Octanoate Metabolism in Healthy Mountaineers After Rapid Ascent to 4559 M
| Autor: | Marco Maggiorini, Raphael N. Vuille-dit-Bille, Werner Schwizer, Luca Emmenegger, Thomas A. Lutz, Tom Sasse, Heiko Fruehauf, Kerstin Spliethoff, Michael Fried, Oliver Goetze, François Verrey, Daniela Meier, Eva E. Kummer |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Hepatology Lipopolysaccharide medicine.diagnostic_test Stomach Gastroenterology Radioimmunoassay Inflammation Metabolism Biology Molecular biology chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Western blot Internal medicine medicine Ghrelin medicine.symptom Hormone |
| Zdroj: | Gastroenterology. 140:S-478 |
| ISSN: | 0016-5085 |
| DOI: | 10.1016/s0016-5085(11)61970-7 |
| Popis: | Background: Bacterial lipopolysaccharide (LPS) is an established animal model to study the innate immune response to gram-negative bacteria mimicking symptoms of infection including reduction of food intake. We previously reported that LPS decreased acyl ghrelin associated with decreased concentrations of circulating ghrelin-O-acyltransferase (GOAT) likely contributing to the anorexigenic effect. In addition, we recently described the prominent expression of the novel anorexigenic hormone, nucleobindin2 (NUCB2)/nesfatin-1 in gastric X/A-like cells co-localized with ghrelin in different pools of vesicles. Aim: To investigate whether LPS would affect gastric and circulating NUCB2/nesfatin-1 concentration. Methods: Ad libitum fed male Sprague-Dawley rats were equipped with an intravenous (IV) catheter placed in the right jugular vein and allowed to recover for four days. LPS was injected intraperitoneally (IP, 100μg/kg) and blood was withdrawn before and at 2, 5, 7 and 24h post injection and processed for NUCB2/nesfatin-1 measurement by radioimmunoassay (n= 5/group). Gastric corpus was collected at different time points to measure NUCB2 mRNA expression by quantitative RT-PCR and NUCB2/nesfatin-1 protein concentration by Western blot. Results: Injection of LPS significantly increased plasma NUCB2/nesfatin-1 concentrations by 55% compared to vehicle at 2h post injection (248.5 ± 45.5 vs. 161.2 ± 14.3 pg/ ml, P |
| Databáze: | OpenAIRE |
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