Acupuncture inhibits liver injury induced by morphine plus acetaminophen through antioxidant system
| Autor: | Jin Mook Kim, Sae-Kwang Ku, Ji Eun Lee, Su Jin Kang, Young Woo Kim, Nam Jun Kim, Suchan Chang, Rong Jie Zhao, Eun Kyung Lee, Young-Joon Lee, Bong Hyo Lee |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pharmacology medicine.disease_cause Superoxide dismutase Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Medicine Liver injury biology business.industry Nitrotyrosine Liver cell digestive oral and skin physiology Malondialdehyde medicine.disease Acetaminophen 030104 developmental biology Complementary and alternative medicine chemistry Anesthesia biology.protein business 030217 neurology & neurosurgery Oxidative stress medicine.drug |
| Zdroj: | European Journal of Integrative Medicine. 8:204-212 |
| ISSN: | 1876-3820 |
| DOI: | 10.1016/j.eujim.2015.12.009 |
| Popis: | Introduction Morphine (MP) and acetaminophen (APAP), widely used-pain relievers and antipyretics, are known to induce hepatotoxicity. Acupuncture, a traditional therapy in Asia, is used for various reasons including detoxification. This study aimed to examine whether acupuncture exerts protective effects against MP + APAP-induced hepatic damage. Methods Male Sprague-Dawley rats received chronic MP, withdrawal, and APAP. Thereafter, blood and liver were taken. Acupuncture was performed once daily. Asparte aminotransferase (AST) and alanine aminotransferase (ALT) levels were measured, and percentages of abnormally decreased-hepatocyte regions, mean liver cell counts, and mean inflammatory cell numbers infiltrated on hepatic parenchyma were examined. In addition, antioxidant effects were evaluated based on liver lipid peroxidation malondialdehyde (MDA) and glutathione (GSH) contents, superoxide dismutase (SOD) and catalase (CAT) activities with the number of immunopositive hepatocytes against nitrotyrosine (NT) as a marker of inducible nitric oxide synthase (iNOS) related-oxidative stresses and 4-hydroxynonenal (4HNE) as a marker of lipid peroxidation. Results Significant elevations of AST and ALT were noted of MP or APAP. They also induced an increase in MDA contents as well as decreases in GSH levels and activities of SOD and CAT activity. A centrolobular decrease in hepatocytes along with degenerative changes of hepatocytes were also observed, and increases of NT and 4HNE immunoreactive hepatocytes were shown. These hepatocellular damages were more severe with the combination of MP + APAP. However, these MP + APAP-induced hepatic damages were significantly inhibited by acupuncture. Conclusion This study suggests that acupuncture may have a protective effect against the MP + APAP-induced hepatic damage through the amelioration of antioxidant defense systems. |
| Databáze: | OpenAIRE |
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