Multicolor Immunofluorescence Staining of Paraffin-Embedded Human Bone Marrow Sections
| Autor: | Simone Pacini, Stefano Mazzoni, Simón Méndez-Ferrer, Francesca M Panvini |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty medicine.diagnostic_test business.industry Human bone Immunofluorescence staining Immunofluorescence Paraffin embedded 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Trephine Antigen retrieval chemistry 030220 oncology & carcinogenesis Medicine Bone marrow business Fixative |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071614242 |
| Popis: | Immunofluorescence is an indispensable method for the identification, localization and study of the expression of target antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections of human bone marrow. However, the procedure shows technical limitations because of the chemical and physical treatments required for sample processing before imaging. Here we describe a revisited protocol to obtain high-resolution images of human bone marrow trephine biopsies, improving the antigen-antibody recognition and preserving the morphology and the architecture of the bone marrow microenvironment. |
| Databáze: | OpenAIRE |
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