Anticancer effect and enhancement of therapeutic potential of Vincristine by extract from aerial parts of Juniperus excelsa on pre-B acute lymphoblastic leukemia cell lines
| Autor: | Somayeh Esmaeili, Mina Darvishi, Ahmad Gharehbaghian, Nasrin Dehghan-Nayeri, Pargol Mashati |
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| Rok vydání: | 2017 |
| Předmět: |
Vincristine
Programmed cell death Health Toxicology and Mutagenesis Biomedical Engineering Caspase 3 Pharmacology Pre-B Acute Lymphoblastic Leukemia 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Artificial Intelligence medicine Cytotoxic T cell MTT assay General Pharmacology Toxicology and Pharmaceutics General Immunology and Microbiology biology General Neuroscience General Medicine biology.organism_classification 0104 chemical sciences 010404 medicinal & biomolecular chemistry Apoptosis 030220 oncology & carcinogenesis Juniperus excelsa General Agricultural and Biological Sciences medicine.drug |
| Zdroj: | Journal of Applied Biomedicine. 15:219-226 |
| ISSN: | 1214-0287 1214-021X |
| DOI: | 10.1016/j.jab.2017.04.002 |
| Popis: | The natural products and conventional chemotherapeutic drugs combination can increase the efficacy of anticancer treatment through their potential synergistic effects and reduce its toxicity. The current study investigates the effect of methanolic extract from aerial parts of Juniperus excelsa on cell death activities induced by vincristine in acute lymphoblastic leukemia cells. Cytotoxic activity of J. excelsa extract and vincristine in Nalm-6 and Reh cells was determined using MTT assay and synergism was evaluated using the CompuSyn software. Apoptosis was assessed by caspase 3 activity assay and flow cytometry. The expression levels of some apoptosis-related genes, Caspase 3, BAX and BCL-2 were determined by Real-time PCR. Statistical analysis was assessed by one-way ANOVA and Tukey's tests. The combined treatment of VCR and J. excelsa extract showed a synergistic cytotoxic effect on both Nalm-6 and Reh cells at low doses of vincristine (CI Our study suggested the combined use of lower doses of VCR and J. excelsa extract promote its effects by apoptosis induction and this combination could potentially decrease the side effects of the drug. |
| Databáze: | OpenAIRE |
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