Multiple elements regulate expression of the cell cycle-regulated thymidylate synthase gene of Saccharomyces cerevisiae

Autor:	Lydia Lee, Evan M. McIntosh, Pak Phi Poon, Reginald Storms, Robin W. Ord
Rok vydání:	1988
Předmět:	Regulation of gene expression Start codon Base pair Gene expression Saccharomyces cerevisiae Genetics Translation (biology) General Medicine Northern blot Biology biology.organism_classification Gene Molecular biology
Zdroj:	Current Genetics. 14:363-373
ISSN:	1432-0983 0172-8083
DOI:	10.1007/bf00419994
Popis:	Expression of the thymidylate synthase gene (TMPI) of Saccharomyces cerevisiae increases during the late G1 phase of the cell cycle. Using a series of gene fusions, which have placed the Escherichia coli lacZ gene under transcriptional and translational control of different portions of the TMPI gene, we have demonstrated the existence of three different regions which are important for expression. One of these regions, which was localized to within 270 base pairs of the translation start codon, is involved in the periodic expression of TMPI transcript. A second region, the deletion of which resulted in reduced levels of TMPI expression, is at least partially encoded by DNA sequences between 270 and 377 base pairs upstream of the translation start codon. A third region, located within the N-terminal 112 codons of the TMPI gene, apparently encodes information involved in a post-translational control mechanism.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a1e25e5dd166f410d5f91f41a6206367 https://doi.org/10.1007/bf00419994 Zobrazit plný text záznamu Full text from SpringerLink