Discovery of c-SNPs in Anemone coronaria L. and Assessment of Genetic Variation

Autor: N. Pollak, A. Shamay, A. Cohen, N. Yonash, J. Fang, U. Lavi
Rok vydání: 2005
Předmět:
Zdroj: Genetic Resources and Crop Evolution. 53:821-829
ISSN: 1573-5109
0925-9864
DOI: 10.1007/s10722-004-6377-5
Popis: Single nucleotide polymorphisms (SNPs) were discovered in 44 cDNA clones from leaves by comparison of the commercial cultivar ‘Mona Lisa‘ with a wild population of Anemone coronaria L. One hundred and fifty five SNPs were discovered with an average frequency of one SNP per 167 bp. Forty nine percent of the SNPs are transitions, 43 are transversions, 26 are heterozygotes and 9% are InDels. Eighty two (68%) of the SNPs located in the ORF, resulted in a change of amino acid while 39 (32%) did not affect the amino acid. Thirty eight SNPs (46%), which change the amino acid, resulted in similar amino acid, while 44 SNPs (54%) resulted in a non-similar amino acid. Nine polymorphic sites were genotyped by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) using genomic DNA. Phylogenetic analysis of 50 individuals from six wild populations and the commercial cultivar revealed high polymorphism within and low polymorphism between A. coronaria L. wild populations. The phylogenetic tree revealed only one clear cluster of the ‘Mona Lisa’ cultivar. All the other individuals from the various wild populations are distributed on different nodes indicating that the genetic variation within the wild populations is high while the commercial cultivar is quite homogenous and genetically different from all other populations.
Databáze: OpenAIRE
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