EARLY EXPRESSION OF INTERFERON-?? INDUCIBLE PROTEIN 10 AND MONOKINE INDUCED BY INTERFERON-?? IN CARDIAC ALLOGRAFTS IS MEDIATED BY CD8+ T CELLS1

Autor: Anil Kapoor, Robert L. Fairchild, Tara M. Engeman, Michael G. Hobart, Shoji Koga, Ken Morita, Evan M. Vapnek
Rok vydání: 2000
Předmět:
Zdroj: Transplantation. 69:1147-1155
ISSN: 0041-1337
DOI: 10.1097/00007890-200003270-00020
Popis: BACKGROUND Our goal was to test the intragraft mRNA expression and production of two chemokines that are potent chemoattractants for antigen-primed T cells, interferon-gamma inducible protein 10 (IP-10) and monokine-induced by IFN-gamma, (Mig), in allogeneic heart grafts. METHODS Syngeneic or allogeneic A/J (H-2a) hearts were heterotopically transplanted to wild-type, CD4-/-, CD8alpha-/-, or IFN-gamma-/- C57BL/6 (H-2b) recipients. To test expression of IP-10 and Mig, grafts were removed 1-8 days posttransplant for RNA isolation and Northern blot analysis. To test the potential recipient leukocyte populations mediating intraallograft expression of IP-10 and Mig, recipients were treated with anti-NK 1.1, anti-CD4, and/or anti-CD8 monoclonal antibodies before transplantation. RESULTS Allogeneic heart grafts transplanted to wild-type, but not IFN-gamma-/-, recipients expressed IP-10 and Mig at day +2 posttransplant that increased thereafter until rejection was completed. Expression of IP-10 and Mig in isografts was low or undetectable. Cardiac allografts from CD8+ T cell depleted, but not NK cell or CD4+ T cell depleted, recipients had low to undetectable expression of IP-10 and Mig on day +2 posttransplant. Similarly, cardiac allografts from CD8-/-, but not CD4-/-, recipients had low to undetectable expression of IP-10 and Mig on day +2 posttransplant. CONCLUSIONS Early intraallograft expression of Mig and IP-10 during primary rejection of cardiac allografts is dependent on the activities of recipient CD8+ T cells.
Databáze: OpenAIRE