Creation of thermostable polypeptide cassettes for amino acid balancing in farm animal rations
| Autor: | D. V. Grishin, Yu A Gladilina, Nikolay N. Sokolov, D. D. Zhdanov, V. S. Pokrovskii, S. S. Aleksandrova, M. V. Pokrovskaya, O. V. Podobed |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification 030102 biochemistry & molecular biology biology Genetically engineered In silico Metabolism biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Amino acid law.invention 03 medical and health sciences 030104 developmental biology chemistry law Recombinant DNA Nucleotide Archaea Thermostability |
| Zdroj: | Applied Biochemistry and Microbiology. 53:688-698 |
| ISSN: | 1608-3024 0003-6838 |
| Popis: | The study of the poultry needs in basic nutrients allowed the development of a scheme for obtaining feed polypeptides (“polypeptide cassettes”) enriched with L-amino acids, which are necessary for the metabolism of birds. The amino acid and nucleotide profiles of about 500 bioinformation sequences of thermostable plant proteins and archaea were studied, on the basis of which candidate sequences were selected. In silico, the amino acid and domain composition of the thermostable polypeptides has been optimized. A library of genetically engineered constructs encoding optimized polypeptides with the necessary composition of L-amino acids irreplaceable for poultry has been created. Primary E. coli producer strains were obtained, and the expression and thermostability of the target polypeptides were studied. |
| Databáze: | OpenAIRE |
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