The use of radioimmunoassay for the detection of polychlorinated dibenzo-p-dioxins in fish samples
| Autor: | J.W. ApSimon, B. K. Afghan, J.P. Sherry, P.W. Albro, R. Wilkinson, L. Collier |
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| Rok vydání: | 1989 |
| Předmět: |
Detection limit
Environmental Engineering Chromatography biology Chemistry Health Toxicology and Mutagenesis Extraction (chemistry) Public Health Environmental and Occupational Health Radioimmunoassay General Medicine General Chemistry Contamination biology.organism_classification Pollution Trout Polychlorinated Dibenzo-p-dioxins Sample size determination Environmental chemistry Environmental Chemistry Fish |
| Zdroj: | Chemosphere. 19:255-261 |
| ISSN: | 0045-6535 |
| Popis: | Because of the increasing numbers of environmental samples requiring analysis for polychlorinated dibenzo-p-dioxin (PCDD) contamination, a need exists for screening techniques, such as radioimmunoassay (RIA), that will facilitate the elimination of PCDD free samples from time-consuming conventional analysis. The RIA for the detection of PCDDs was interfaced with an extraction and cleanup procedure, and its performance was assessed using extensively and minimally cleaned-up Lake Trout samples. Sample size appeared to influence assay performance, probably because of its relationship to the specific detection limit: the larger the sample size that can be analyzed without adversely affecting the amount of TCDD detectable, the lower will be the specific detection limit. However, larger than optimal samples narrowed the assay's working range, adversely affected dose response, and raised the detection limit. The working range of the assay results from a compromise between the required degree of cleanup and sample size. |
| Databáze: | OpenAIRE |
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