Implementing a fully automated high-throughput strategy for blood donor genotyping
| Autor: | Hadassa Campos Santos, José Eduardo Krieger, José Eduardo Levi, Carla Luana Dinardo, A. J. O. Gallucci, Ester Cerdeira Sabino, Marcia Regina Dezan, V. G. Rocha, Helves Domingues, V.B. Oliveira, Alfredo Mendrone-Junior, Juliana Vieira dos Santos Bianchi, A. C. Pereira, Francisco C. A. Gomes |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Nucleic acid methods
030204 cardiovascular system & hematology Biology Bioinformatics 03 medical and health sciences Red blood cell 0302 clinical medicine Blood donor medicine.anatomical_structure Fully automated medicine General Earth and Planetary Sciences Genotyping Throughput (business) 030215 immunology General Environmental Science |
| Zdroj: | ISBT Science Series. 12:357-364 |
| ISSN: | 1751-2816 |
| DOI: | 10.1111/voxs.12354 |
| Popis: | Background High-throughput molecular assays are essential tools for the search of rare blood donors. Aims and methods To evaluate a fully automated workflow designed for genotyping blood donors’ red blood cell (RBC) and platelets (PTL) antigens using the OpenArray Real-time PCR system. Results 5487 blood donors were genotyped using the proposed strategy in two steps: (1) nucleic acid purification in the QIASymphony® equipment and (2) genotyping of the most important RBC and PTL antigens by a customized assay designed for the OpenArray Real-time PCR system. 142 662 single nucleotide polymorphisms were genotyped with 99·6% of accuracy in 15 work-days. Software was created for the purpose of data interfacing and organization of a database to be used in a regular basis to find compatible blood for alloimmunized patients. Conclusions The proposed fully automated genotyping strategy is accurate, fast and suitable for meeting the needs of a laboratory of molecular immunohematology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|