Proteomic Analysis through Adventitious Rooting of Pinus radiata Stem Cuttings with Different Rooting Capabilities
| Autor: | Patricia L. Sáez, Carolina Alvarez, Luis Valledor, Darcy Ríos, Manuel Sánchez-Olate |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification Vegetative reproduction Pinus radiata Radiata Protein level General Medicine Biology Meristem biology.organism_classification 01 natural sciences 03 medical and health sciences Cutting 030104 developmental biology chemistry Cell wall organization Auxin Botany 010606 plant biology & botany |
| Zdroj: | American Journal of Plant Sciences. :1888-1904 |
| ISSN: | 2158-2750 2158-2742 |
| DOI: | 10.4236/ajps.2016.714174 |
| Popis: | In forest production systems, vegetative propagation of elite clones through adventitious rooting is a common practice. In Chile, adventitious rooting is the main methodology for vegetative reproduction of Pinus radiata. However, the capability of produce adventitious roots in gymnosperms decreases with aging. While it is true that some efforts have been made to identify markers or/and regulators of the aging process and adventitious rooting, molecular mechanisms that regulate both processes are scarcely known, especially at protein level. This research evaluated qualitative and quantitative changes in protein accumulation during the adventitious rooting process of P. radiata stem cuttings, with different rooting capabilities. Beside, an analysis of morpho-anatomical changes was performed in stem cuttings with high and low rooting capabilities, during the adventitious rooting process. It was observed that juvenile 1-year-old stem cuttings rooted in a 100%, while aged stem cuttings (3-year-old) presented only a 20% of rooting. According to the results of differential protein accumulation, univariate and multivariate analysis indicated that in total, 114 and 89 proteins were differentially accumulated in juvenile and aged cuttings, respectively. Also, identification of such proteins showed the presence of proteins related to cell wall organization and the presence of a protein related with proper distribution of auxin PIN transporter, both key in the new meristem formation process during adventitious rooting. |
| Databáze: | OpenAIRE |
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