Oleanolic Acid Ameliorates Benign Prostatic Hyperplasia by Regulating PCNA-Dependent Cell Cycle Progression In Vivo and In Vitro
| Autor: | Hyo Jung Kim, Bo-Ram Jin, Hyo-Jin An, Se-Yun Cheon |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.drug_class
Pharmaceutical Science Pharmacology urologic and male genital diseases 01 natural sciences Analytical Chemistry chemistry.chemical_compound Downregulation and upregulation Drug Discovery medicine Oleanolic acid biology urogenital system 010405 organic chemistry business.industry Organic Chemistry Hyperplasia Cell cycle Androgen medicine.disease 0104 chemical sciences Proliferating cell nuclear antigen 010404 medicinal & biomolecular chemistry Complementary and alternative medicine chemistry Dihydrotestosterone Finasteride biology.protein Molecular Medicine business medicine.drug |
| Zdroj: | Journal of Natural Products. 83:1183-1189 |
| ISSN: | 1520-6025 0163-3864 |
| DOI: | 10.1021/acs.jnatprod.9b01210 |
| Popis: | Oleanolic acid (OA) is a natural, biologically active pentacyclic triterpenoid found in Cornus officinalis. Although C. officinalis and OA have antiproliferative actions, the effects and mechanisms of OA in benign prostatic hyperplasia (BPH) are unclear. We examined the effect of OA in an animal model of testosterone-induced BPH. Male rats were injected with testosterone propionate with or without OA. The inhibitory effect of OA on BPH-1 cells was determined in vitro. Rats with BPH exhibited outstanding BPH symptoms, including prostatic enlargement, upregulated dihydrotestosterone and 5α-reductase 2 levels, and histological changes. Compared with the BPH group, the OA group showed fewer pathological alterations and regular androgen events. OA inhibited prostate cell proliferation by downregulating the expression of proliferating cell nuclear antigen (PCNA) and cell cycle markers in BPH-induced animals. This indicated that OA has superior therapeutic effect in the BPH animal model than finasteride. In vitro studies demonstrated upregulation of PCNA and cell cycle proteins, whereas OA clearly reduced this upregulation. Thus, OA may inhibit the development of BPH by targeting cell cycle progression markers. These suggest that OA is a potential agent for BPH treatment. |
| Databáze: | OpenAIRE |
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