Detection ofToxoplasmaDNA in the Peripheral Blood of HIV-Positive Patients with Neuro-opportunistic Infections by a Real-Time PCR Assay

Autor:	Andres Felipe Zea, Jorge Enrique, Anilza Bonelo, Beatriz Parra, Natalia Basto, Carlos A. Pardo, Nestor Cardona
Rok vydání:	2011
Předmět:	Cerebral toxoplasmosis Real-time polymerase chain reaction Gene targets Immunology Pcr assay Human immunodeficiency virus (HIV) medicine TaqMan In patient Biology medicine.disease_cause Peripheral blood
Zdroj:	Journal of Neuroparasitology. 2:1-6
ISSN:	2090-2352 2090-2344
DOI:	10.4303/jnp/n110402
Popis:	We determine if peripheral blood sample could be used for diagnosis of cerebral toxoplasmosis by a real-time PCR assay and we analyzed the clinical and laboratory findings in 22 patients with confirmed cerebral toxoplasmosis compared to 27 patients with other neuro- opportunistic infections. We compared two gene targets (B1 and RE) in the Taqman-PCR real-time assay. Efficiency values were also calculated. We found that 18.8% (4/22) of cases with cerebral toxoplasmosis and 7.4% (2/27) of patients with other neuroinfections had positive results with the Taqman PCR assay. The mean number of parasites was 67.7 (SD 69.0) tachyzoites/mL in patients with cerebral toxoplasmosis and of 31.8 (SD 6.3) tachyzoites/mL in other neuroinfections. Clinical symptoms as headache were significantly less frequent and focal neurological symptoms were significantly more frequent in cerebral toxoplasmosis than in other neuroinfections. In our conditions, real- time PCR on peripheral blood samples was not useful for diagnosis of cerebral toxoplasmosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a16bc8a4cc36102d6cc4bea3a5e6701e https://doi.org/10.4303/jnp/n110402 Zobrazit plný text záznamu