Oxygen Electrode With Pyrolyzed CoTMPP Catalyst: Application in Glucose Biosensor
| Autor: | Plamen Atanasov, S. Gamburzev, Ebtisam Wilkins |
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| Rok vydání: | 1997 |
| Předmět: |
Working electrode
biology Chemistry Biochemistry (medical) Clinical Biochemistry Inorganic chemistry Analytical chemistry chemistry.chemical_element Electrocatalyst Biochemistry Oxygen Amperometry Analytical Chemistry law.invention law Electrochemistry biology.protein Glucose oxidase Biosensor Oxygen sensor Clark electrode Spectroscopy |
| Zdroj: | Analytical Letters. 30:503-514 |
| ISSN: | 1532-236X 0003-2719 |
| DOI: | 10.1080/00032719708001797 |
| Popis: | Amperometric oxygen sensors employing non-platinum electrocatalyst made from pyrolyzed cobalt-tetramethoxy-phenylporphyrin (CoTMPP) are described. A technique for the preparation of an oxygen working electrode with catalyst from a pressed matrix (tablet) is presented. This technique allows manufacturing numerous electrodes from the same pressed tablet, all of which demonstrate good reproducibility of their parameters. Glucose biosensors are constructed using this oxygen electrode as a basic transducer for amperometric detection of oxygen consumption. The enzyme (glucose oxidase) is held in a micro-bioreactor (face side closed by a Silastic coated polycarbonate membrane) in which the oxygen sensor is immersed. Linearity of the sensor response to glucose concentrations up to 16 mM is reported. Sensors demonstrated a stable signal (varying within ±5% of its average value) during 15 days of continuous in vitro glucose monitoring at 37°C. |
| Databáze: | OpenAIRE |
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