Molecular linkage mapping in rye (Secale cereale L.)

Autor: J. P. Gustafson, M. Xie, Xue-Feng Ma, M. A. Rodriguez Milla, P. G. Goicoechea, Michael K. Wanous, K. Houchins, Z. Wang
Rok vydání: 2001
Předmět:
Zdroj: Theoretical and Applied Genetics. 102:517-523
ISSN: 1432-2242
0040-5752
DOI: 10.1007/s001220051676
Popis: A rye linkage map containing clones from rye, wheat, barley, oat and rice genomic and cDNA libraries, known-function genes and microsatellite markers, was created using an F2 population consisting of 110 F2-derived F3 families. Both co-dominant and dominant markers were added to the map. Of all probes screened, 30.8% were polymorphic, and of those polymorphic 79.3% were mapped. The current map contains 184 markers present in all seven linkage groups covering only 727.3 cM. This places a marker about every 3.96 cM on average throughout the map; however, large gaps are still present. The map contains 60 markers that have been integrated from previous rye maps. Surprisingly, no markers were placed between the centromere and C1–1RS in the short arm of 1R. The short arm of chromosome 4 also lacked an adequate number of polymorphic markers. The population showed a remarkable degree of segregation distortion (72.8%). In addition, the genetic distance observed in rye was found to be very different among the maps created by different mapping populations.
Databáze: OpenAIRE
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