Tumor necrosis factor-α-secreting CD16+ antigen presenting cells are effectively removed by granulocytapheresis in ulcerative colitis patients

Autor:	Antonio Torres-Gomez, Valle García-Sanchez, Rosario Jimenez-Moreno, Carlos Pérez-Seoane, Juan F De Dios, Concepcion Herrera-Arroyo, Joaquin Sanchez-Garcia, Miguel A. Alvarez‐Rivas, Josefina Serrano, Juana Serrano-Lopez
Rok vydání:	2010
Předmět:	Myeloid Hepatology medicine.diagnostic_test business.industry Gastroenterology Leukapheresis CD16 medicine.disease Ulcerative colitis Flow cytometry medicine.anatomical_structure Immunology Medicine Tumor necrosis factor alpha Colitis business Antigen-presenting cell
Zdroj:	Journal of Gastroenterology and Hepatology. 25:1869-1875
ISSN:	0815-9319
DOI:	10.1111/j.1440-1746.2010.06377.x
Popis:	Background and Aim: In human blood, two main subsets of antigen-presenting-cells (APCs) have been described: plasmocytoid dendritic cells (pDC) and myeloid dendritic cells (mDC) which are further subdivided in CD11c-mDC and CD16-mDC DC. In ulcerative colitis patients (UC) peripheral blood APCs express significant levels of the activation and lack immature-tolerogeneic APCs. Adacolumn selective granulocytapheresis (GCAP) has been associated with clinical efficacy in patients with UC. In the present study we sought the effect of sequential GCAP procedures in peripheral blood APCs in patients with UC and the effect on soluble cytokines. Methods: We used multiparametric flow cytometry to quantify peripheral blood APCs and serum cytokines in 210 samples obtained from seven patients with steroid-dependent or steroid resistant UC undergoing GCAP treatment. Samples were drawn before, after 30 and 60 min of each session. Results: Each GCAP session resulted in a dramatic tenfold reduction of peripheral blood CD16-mDC (P
Databáze:	OpenAIRE
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