High Throughput Lc-Ms/Ms Method for the Quantitation of Emtricitabine in Human Plasma by Solid Phase Extraction Using 96 Well Plate Format
| Autor: | Tangudu Nagabhusana Rao, Guntuku Girija Sankar, Lade Jyothi Rani |
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| Rok vydání: | 2016 |
| Předmět: |
Analyte
Chromatography 010401 analytical chemistry Analytical chemistry General Medicine Mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences 0104 chemical sciences Triple quadrupole mass spectrometer 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry Ionization Phase (matter) Ammonium formate Solid phase extraction Methanol |
| Zdroj: | British Journal of Pharmaceutical Research. 11:1-10 |
| ISSN: | 2231-2919 |
| DOI: | 10.9734/bjpr/2016/24231 |
| Popis: | High-throughput Liquid chromatography–mass spectrometry method has been developed and validated for the quantification of Didanosine in human plasma using Zidovudine as an internal standard (ISTD). Following solid phase extraction (SPE) in 96 well plate format, the analyte and ISTD were run on Inertsil ODS-3, 4.6 X 100 mm, 5 μm column using an isocratic mobile phase consisting of 10mM Ammonium formate buffer (pH 6.4 ± 0.5): Methanol 10:90 v/v. The precursor and product ions of the drugs were monitored on a triple quadrupole instrument operated in the positive ionization mode. The method was validated over a concentration range of 1.01 to 2002.18 ng/mL with relative recoveries ranging from 92.4 to 96.4%. The inter batch precision (%CV) across three validation runs was ≤ 8.9%. The Inter batch percent relative errors (%RE) determined at four QC levels (LLOQ, LQC, MQC and HQC) was between -7.7 to 4.9%. According to the validated results, the proposed method was found to be specific, accurate, precise and high throughput method and could be used for the estimation of Didanosine in human plasma and can be applied for the routine analysis. |
| Databáze: | OpenAIRE |
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