Dynamic changes of NK and LAK activity in head and neck cancer patients

Autor:	Hirokazu Hariki, Mitsunobu Sato, Shigehiro Seo, Masanori Ikawa, Takashi Bando, Masato Okamoto, Yoshiaki Yura, Tetsuo Yanagawa, Hirofumi Kasetani, Hideo Yoshida, Ryoji Kaji
Rok vydání:	1992
Předmět:	medicine.diagnostic_test business.industry Immunology Head and neck cancer Recurrent disease Medicine chemical and pharmacologic phenomena business medicine.disease Molecular biology Peripheral blood Flow cytometry
Zdroj:	Japanese Journal of Oral & Maxillofacial Surgery. 38:229-235
ISSN:	2186-1579 0021-5163
DOI:	10.5794/jjoms.38.229
Popis:	Natural Killer (NK) and lymphokine-activated killer (LAK) activity as well as surface markers of peripheral blood lymphocytes (PBL) in head and neck cancer patients are investigated with regard to the prognosis of patients.Patients (n=31) were grouped as follows:(1) untreated (UT) group (n=29), (2) treated, disease-free (TDF) group (n=21), and (3) group with recurrent disease (RD;n=6).NK and LAK activities were assayed by 51 Cr release method using K-562 cells in NK assay or Daudi cells in LAK assay as targets. Also LAK induction rate from PBL by interleukin-2 (IL-2) was measured.Analysis of surface markers of PBL (Leu 2, Leu 3, Leu 4, Leu 7, Leu 11, HLA-DR, and M1) including two color analysis (Leu 4×HLA-DR, Leu 2×Leu 15, Leu 3×Leu 8, and Leu 7×Leu 11) were performed using flow cytometry system.NK activity (P
Databáze:	OpenAIRE
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