TECHNOLOGY OF REGENERATION OF THE BONE TISSUE IN A ROTATIONAL BIOREACTOR: FLUID FLOW SIMULATION AND LASER-INDUCED FLUORESCENCE DIAGNOSTICS
| Autor: | E. O. Tsibulskaya, V. L. Ganimedov, N. A. Maslov, P. M. Larionov |
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| Rok vydání: | 2020 |
| Předmět: |
Bone growth
Scaffold Materials science Mechanical Engineering 02 engineering and technology Condensed Matter Physics Bone tissue 01 natural sciences Fluorescence 010305 fluids & plasmas chemistry.chemical_compound 020303 mechanical engineering & transports medicine.anatomical_structure 0203 mechanical engineering chemistry Mechanics of Materials 0103 physical sciences Polycaprolactone Shear stress Bioreactor medicine Laser-induced fluorescence Biomedical engineering |
| Zdroj: | Journal of Applied Mechanics and Technical Physics. 61:776-786 |
| ISSN: | 1573-8620 0021-8944 |
| DOI: | 10.1134/s0021894420050120 |
| Popis: | A technology of bone tissue cultivation on a thin scaffold in a rotational bioreactor is developed. An optimal regime of cell material cultivation is determined for testing the method, and an optical method for diagnostics of the bone growth dynamics during its cultivation is found. Numerical simulations of the fluid flow in the bioreactor make it possible to significantly simplify the medical experiment and to choose the optimal values of the rotation frequencies and shear stresses acting on the cell material placed on the scaffold. The optical diagnostics of the scaffold samples in the course of dynamic cultivation in the bioreactor is performed by the method of laser-induced fluorescence. An algorithm based on the principal component analysis is applied to analyze the spectral data; as a result, the spectra of excitation and fluorescent emission of the basic fluorescent substances in the sample (tyrosine and tryptophan amino acids, structural protein (collagen), and fluorescent structures of polycaprolactone) are calculated. It is found that the contribution of the component corresponding to collagen in the samples increases in the process of dynamic cultivation, which testifies to effective formation of the extracellular matrix of the bone. |
| Databáze: | OpenAIRE |
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