Optimization of supercritical fluid extraction-gas chromatography of methylmercury in marine samples
| Autor: | M. Miguéns-Rodríguez, R. Cela-Torrijos, R.A. Lorenzo-Ferreira, A.M. Carro-Díaz |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Chromatography
Chemistry Organic Chemistry Kinetics Extraction (chemistry) Supercritical fluid extraction Analytical chemistry Hydrochloric acid General Medicine Factorial experiment Biochemistry Analytical Chemistry Electron capture detector chemistry.chemical_compound Gas chromatography Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography A. 750:191-199 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/0021-9673(96)00346-9 |
| Popis: | Two-level factor designs were used to optimize the supercritical fluid extraction (SFE) of methylmercury (MeHg) in marine samples, which were subsequently analysed by gas chromatography-electron capture detection (GC-ECD). Several variables potentially affect to extraction efficiency and kinetics. The factors studied were: CO2 flow-rate and density, extraction cell temperature, static extraction time, nozzle and trap temperature, amount of hydrochloric acid and contact time between the acid and the sample before to extraction. The extraction kinetics was studied in all experiments by splitting extracts at 2, 7, 17 and 37 min. The results suggest that only the extraction cell temperature is statistically significant. The optimized SFE procedure to extract MeHg was validated by means of three available reference materials having certified methylmercury content. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect