| Autor: |
Jian-gang Gao, Li Dan, Gong Jie, Tang Yimiao, Gao Shiqing, Liu Yongjie, Wei-bing Yang, Zhang Fengting, Zhao Changping, Pang Binshuang, Yong-bo Wang, Chen Zhaobo, Chen Xianchao |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
Background: Known as the prerequisite component for the heterosis breeding system, the male sterile line determined the hybrid yield and seed purity. Therefore, a deep understanding of the mechanism and gene network that leads to male sterility is crucial. BS366, a temperature-sensitive genic male sterile (TGMS) line, were male sterile under cold condition (12℃ with 12 h daylight) but fertile under normal temperature (20℃ with 12 h daylight). Results: During the meiosis, BS366 was defective in forming tetrad and dyad due to the abnormal cell plate. During the pollen development, unusual vacuolated pollen that cannot accumulate starch grains at the binucleate stage was also observed. Transcriptome analysis revealed that genes involved in the meiotic process like sister chromatid segregation and microtubule-based movement were repressed, while genes involved in DNA and histone methylation were induced in BS366 under cold condition. MethylRAD was used for a reduced DNA methylation sequencing for BS366 under both cold and control conditions. The differentially methylated sites (DMSs) located in the gene region were mainly involved in carbohydrate and fatty acid metabolism, lipid metabolism, and transport. Genes differentially and methylated were mainly involved in cell division. Conclusions: These results indicated that methylation of genes involved in carbon metabolism or fatty acid metabolism would contribute to the male sterility in BS366, which will provide a novel insight into the molecular mechanism of wheat male sterility. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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