Exoproteinases of the Oomycete Phytophthora infestans
Autor: | Ievleva Ev, Valueva Tatyana A, N. G. Gerasimova, Ozeretskovskaia Ol, E. L. Gvozdeva |
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Rok vydání: | 2004 |
Předmět: |
Oomycete
chemistry.chemical_classification biology fungi Subtilisin food and beverages Matrix metalloproteinase biology.organism_classification Trypsin Applied Microbiology and Biotechnology Biochemistry Microbiology Serine Enzyme chemistry Phytophthora infestans Extracellular medicine medicine.drug |
Zdroj: | Applied Biochemistry and Microbiology. 40:165-169 |
ISSN: | 0003-6838 |
Popis: | When grown in a medium containing heat-stable potato tuber proteins, the oomycete Phytophthora infestans (Mont.) de Bary produces a set of exoproteinases active at neutral and mildly basic pH values. These extracellular proteinases have been shown (by SDS-PAGE in the presence of gelatin) to include at least six components differing in molecular weight. Inhibitory analysis and studies of the effects of the enzymes on various synthetic substrates show that the culture liquid of P. infestans contains mainly serine proteinases (specific for trypsin and subtilisin) and metalloproteinases. Their activity is suppressed by proteinase-inhibiting proteins from potato tubers. It is suggested that exoproteinases of P. infestans may be the metabolic target for natural proteinase inhibitors from potato. |
Databáze: | OpenAIRE |
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