One-pot preparation of boronic acid and PEG bi-functionalized silica particles for separation and purification of catecholamine from rat serum

Autor:	Lei Sun, Shian Zhong, Yan Wang, Shaotong Li, Bingqing Yu
Rok vydání:	2015
Předmět:	Sorbent Chromatography Chemistry Extraction (chemistry) General Chemistry Catalysis Contact angle chemistry.chemical_compound Adsorption PEG ratio Materials Chemistry Solid phase extraction Boronic acid Protein adsorption
Zdroj:	New Journal of Chemistry. 39:8848-8854
ISSN:	1369-9261 1144-0546
DOI:	10.1039/c5nj01965f
Popis:	A facile one-pot preparation of aminophenylboronic acid (APBA) and poly(ethyleneglycol) (PEG) bi-functionalized silica particles named SiO2@APBA@PEG by the aid of epoxy silanes is described in this work. The as-prepared particles could not only selectively extract the catecholamine through boronate binding but could also effectively resist protein adsorption using PEG brushes; FT-IR spectra and contact angle measurements proved that APBA and PEG groups were successfully introduced into the surface of silica. The maximum adsorption capacities towards dopamine (DA) and levodopa (L-DOPA) were found to be 254.45 and 104.71 μmol g−1, respectively. The as-prepared material was used as a solid phase extraction (SPE) sorbent for the extraction of catecholamine from rat serum. The recoveries of DA and L-DOPA were 95 ± 3.21% and 93 ± 3.57%, meanwhile, proteins were eliminated. The obtained results indicated that the SiO2@APBA@PEG particles were qualified for efficient purification of catecholamine from complex biological samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a1040b722c3f5aab38920f7b354c3645 https://doi.org/10.1039/c5nj01965f Zobrazit plný text záznamu