Partial Purification and Characterization of the mRNA for α-Amylase from Barley Aleurone Layers

Autor:	Thomas J. Mozer
Rok vydání:	1980
Předmět:	Gel electrophoresis Differential centrifugation Messenger RNA Lysis biology Physiology Plant Science chemistry.chemical_compound medicine.anatomical_structure Reticulocyte chemistry Biochemistry Aleurone Genetics medicine biology.protein Amylase Gibberellic acid
Zdroj:	Plant Physiology. 65:834-837
ISSN:	1532-2548 0032-0889
DOI:	10.1104/pp.65.5.834
Popis:	The poly(A)-containing mRNA from barley aleurone layers pretreated with gibberellic acid has been purified by phenol-chloroform extraction and repeated oligo[d(pT)]-cellulose chromatography. This RNA has been translated in both the wheat germ and reticulocyte lysate in vitro translation systems with greater than 50% of the synthesized protein being α-amylase. The mRNA for α-amylase has been further purified by dimethylsulfoxide-formamide-sucrose density gradient centrifugation and by gel electrophoresis. By these methods, its molecular weight has been determined to be 580,000.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a0f2efc3d2fb2ca47ce15b971b7844c4 https://doi.org/10.1104/pp.65.5.834 Zobrazit plný text záznamu