Carboxyl-terminus of the amyloid protein precursor and ERβ are required for estrogenic effect in activating mitogen-activated protein kinase
| Autor: | Yhun Yhong Sheen, Su J. Seo, Youn S. Song, Sae H. Min, Jung S. Cho, Yong K. Kim, Dae Y. Hwang, Su H. Lee, Hwa J. Lim, Chul J. Lim, Hye K. Park |
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| Rok vydání: | 2004 |
| Předmět: |
MAPK/ERK pathway
medicine.diagnostic_test medicine.drug_class p38 mitogen-activated protein kinases Estrogen receptor General Medicine Transfection Biology Molecular biology Western blot Estrogen Mitogen-activated protein kinase mental disorders Genetics medicine biology.protein Protein kinase A hormones hormone substitutes and hormone antagonists |
| Zdroj: | International Journal of Molecular Medicine. |
| ISSN: | 1791-244X 1107-3756 |
| Popis: | Estrogen influences the processing of the amyloid beta precursor protein (APP) in the pathogenesis of Alzheimer's disease, and this effect is mediated by estrogen receptors (ERs) in activating mitogen-activated protein kinase (MAPK)-signaling pathway. To test whether the estrogenic effect on both carboxyl-terminal amino acid fragment (C-terminal) of APP (APP-C105)- and ERbeta-mediated MAPK activation in in vitro, two hybrid genes containing each human ERbeta and APP-C105 gene fused to the neuron-specific enolase (NSE) promoter were constructed and were transfected to the neuronal SK-N-MC cells. Western blot shows that the activation of JNK-signaling pathway, but not p38 and ERK, is dependent on ERbeta through estrogen treatment and APP-C105 is also mediated through estrogen in activating MAPK-signaling pathway. The results suggest that ERbeta and APP-C105 derived from APP are necessary for estrogenic effect in activating MAPK-signaling pathway. |
| Databáze: | OpenAIRE |
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