The biological response of hairy roots to O2 levels in bioreactors
Autor: | Barbara E. Wyslouzil, Yoojeong Kim, Erbay Yigit, Kristin K. Wobbe, Pamela J. Weathers |
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Rok vydání: | 1999 |
Předmět: | |
Zdroj: | In Vitro Cellular & Developmental Biology - Plant. 35:286-289 |
ISSN: | 1475-2689 1054-5476 |
Popis: | The efficient exchange of gases between roots and their environment is one of the biggest challenges in bioreactor design for transformed root cultures. Gas-phase reactors can alleviate this problem as well as provide a new tool for studying the biological response of roots and other differentiated tissues to changes in the gas phase composition. In our comparison of liquid- and gas-phase reactors, roots grown in liquid (shake flasks or bubble column reactors) are shown to be under hypoxic stress. Roots grown in a gas-phase reactor (nutrient mist), while not hypoxic, produced 50% less biomass. These results suggest that the response of the tissues to gas phase composition are complex and need further study. |
Databáze: | OpenAIRE |
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