Gender Identification of Grasshopper Sparrows Comparing Behavioral, Morphological, and Molecular Techniques

Autor:	Petra Bohall Wood, Frank K. Ammer, Roger J. McPherson
Rok vydání:	2008
Předmět:	Sparrow Ecology biology biology.organism_classification Evolutionary biology biology.animal Agarose gel electrophoresis Biological dispersal Animal Science and Zoology Identification (biology) Primer (molecular biology) Grasshopper Ecology Evolution Behavior and Systematics Ammodramus
Zdroj:	The Wilson Journal of Ornithology. 120:221-225
ISSN:	1938-5447 1559-4491
DOI:	10.1676/07-002.1
Popis:	Correct gender identification in monomorphic species is often difficult especially if males and females do not display obvious behavioral and breeding differences. We compared gender specific morphology and behavior with recently developed DNA techniques for gender identification in the monomorphic Grasshopper Sparrow (Ammodramus savannarum). Gender was ascertained with DNA in 213 individuals using the 2550F/2718R primer set and 3% agarose gel electrophoresis. Field observations using behavior and breeding characteristics to identify gender matched DNA analyses with 100% accuracy for adult males and females. Gender was identified with DNA for all captured juveniles that did not display gender specific traits or behaviors in the field. The molecular techniques used offered a high level of accuracy and may be useful in studies of dispersal mechanisms and winter assemblage composition in monomorphic species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a0d3b65f2dcca81ec4118ae4e8981171 https://doi.org/10.1676/07-002.1 Zobrazit plný text záznamu Plný text ve formátu PDF