Optimization of genetic constructs for high-level expression of the darbepoetin gene in mammalian cells

Autor:	K. Yu. Kazachenko, R. A. Khamitov, Elena Sautkina, A. A. Nurbakov, D. G. Kozlov, R. R. Shukurov, Yu. A. Seryogin
Rok vydání:	2014
Předmět:	Genetics Expression vector Synthetic gene Chinese hamster ovary cell Gene expression Codon optimization Computational biology High level expression Biology Transient transfection Applied Microbiology and Biotechnology Biochemistry Gene
Zdroj:	Applied Biochemistry and Microbiology. 50:802-811
ISSN:	1608-3024 0003-6838
Popis:	Genetic constructs were designed in order to optimize darbepoetin production in CHO cells. They are characterized by a higher level of structural optimization of the darbepoetin gene, a higher gene dose, and the selection of promoter elements that have never been used before for this purpose. A transient transfection of CHO cells by the obtained constructs was performed. It was shown that each of the variable factors in the constructs influenced darbepoetin gene expression. A construct containing a doubled dose of the darbepoetin synthetic gene with optimized codon composition under the control of the CMV-EF1α chimerical promoter was proved to be the most efficient.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a0d31c952b1c8efb384476edb099837d https://doi.org/10.1134/s0003683814090051 Zobrazit plný text záznamu Plný text ve formátu PDF