Bioassay-Guided Isolation, Identification of Compounds from Sterculia diversifolia and Investigation of Their Anti-Glycation and Antioxidant Activities

Autor: Fazle Rabbi, Amir Zada, Muhammad Sohail, Waheed Ali Shah, Amna Nisar, Saifullah Khan Khalil
Rok vydání: 2020
Předmět:
Zdroj: Pharmaceutical Chemistry Journal. 53:1137-1144
ISSN: 1573-9031
0091-150X
DOI: 10.1007/s11094-020-02137-7
Popis: The current study was conducted to isolate and identify bioactive compounds from Sterculia diversifolia G. Don and to investigate their anti-glycation and antioxidant activities. Stem bark and leaves of S. diversifolia were dried, powdered, and then extracted and fractionated using various organic solvents. The ethyl acetate fraction was selected to isolate the active compounds by column chromatography. The structures of these compounds were elucidated by mass spectrometry, 1D and 2D NMR spectroscopy. Thus, gossypetin (1), taxifolin (2), methyl 4-hydroxycinnamate (3), and β-sitosterol-D-glucoside (4) were isolated and identified. The ethyl acetate fractions of S. diversifolia stem bark and leaves exhibited significant anti-glycation activity with percentage inhibition of 48.72 and 49.48%, respectively. Total flavonoid content in both stem bark and leaves was higher in the ethyl acetate and DCM fractions, respectively. Taxifolin and gossypetin showed percentage inhibition of 48.74 and 46.98%, respectively. Almost all fractions and MESD of both stem bark and leaves possess significant antioxidant activity except n-hexane fraction of leaves, while isolated compounds taxifolin and gossypetin showed 94.31 and 93.96% inhibition, respectively. Taxifolin (2) and gossypetin (1) produced maximum anti-glycation and antioxidant effects in both assays. It has been concluded that taxifolin and gossypetin could be promising antidiabetic agents.
Databáze: OpenAIRE
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