COMPARATIVE STABILITY OF POLIOVIRUS, HEPATITIS A VIRUS AND THEIR RNA TO THE IMPACT OF ULTRAVIOLET RADIATION
| Autor: | Doskina Tv, Aleksandr E. Nedachin, V. A. Dolgin, Dmitrieva Ra |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Infectivity Hepatitis viruses Health Toxicology and Mutagenesis Poliovirus 030106 microbiology Public Health Environmental and Occupational Health RNA General Medicine 010501 environmental sciences Biology medicine.disease_cause medicine.disease 01 natural sciences Pollution Virology Hepatitis a virus Reverse transcriptase Reverse transcription polymerase chain reaction 03 medical and health sciences medicine Ultraviolet radiation 0105 earth and related environmental sciences |
| Zdroj: | Hygiene and sanitation. 98:1240-1244 |
| ISSN: | 2412-0650 0016-9900 |
| Popis: | Introduction. The results of experimental studies on the comparative assessment of the effects of various doses of UV radiation on the survival of poliovirus type I LSc2ab, phage MS-2, hepatitis A viruses and their RNA in tap water are presented. Material and methods. Poliomyelitis viruses of type I strain LSc2ab (PV), viruses of hepatitis A, strain HAS-15 (HAV), phages MS-2, free RNA isolated from hepatitis viruses and poliomyelitis were introduced into model reservoirs with dechlorinated Moscow tap water. Water samples were taken from each tank and subjected to ultraviolet irradiation (UVR) with a wavelength of 254 nm with doses of 25, 40, 60, 80 and 100 mJ/cm2. PV titration was performed on a BGM monkey kidney cell transplant line; MS-2 phages were determined by the agar layer method using the E. coli K12F + Str. detector; determination of PV RNA and HAV was carried out on the Rotor GeneTM 6000 amplifier in RT-PCR reaction in real-time using appropriate test systems. Extraction and isolation of RNA from samples of PV and HAV were also performed using reagent kits of domestic and foreign production. Results. Ultraviolet irradiation in doses from 25 to 100 mJ/cm2 was shown to have a pronounced inhibitory effect on phages MS-2 and PV, determined by traditional methods in accordance with the methodological guidelines MUK 4.2.1018-01 and MUK 4.2.2029-05. At UVR doses of 80 and 100 mJ/cm2, complete inactivation of MS-2 and PV phages in water was noted. At the same time, these same doses of UVR had a less inhibitory effect on PVA, HAV RNA, as well as on isolated free PVA RNA/X and HAV, which were more stable and continued to be determined by RT-PCR in water at all doses of UVR, including 80 and 100 mJ/cm2. Conclusion. If only RNA viruses are detected in the treated drinking water and there are no other direct or indirect indices of viral contamination, it is impossible to unambiguously judge the extent of the potential epidemic hazard of the water body. This requires the development of reliable additional tests confirming the infectivity of viruses, determined only by RNA or DNA markers. |
| Databáze: | OpenAIRE |
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