Different Toxicity of the Novel Bacillus thuringiensis (Bacillales: Bacillaceae) Strain LLP29 Against Aedes albopictus and Culex quinquefasciatus (Diptera: Culicidae)
| Autor: | Ivan Gelbič, Lingling Zhang, Enjiong Huang, Xiong Guan, Lei Xu, Zhaoxia Liu, Baozhen Tang, Zhipeng Huang, Tianpei Huang |
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| Rok vydání: | 2013 |
| Předmět: |
Bacillaceae
Aedes albopictus Ecology biology Brush border medicine.diagnostic_test Toxin fungi General Medicine biology.organism_classification medicine.disease_cause Molecular biology Bacillales Culex quinquefasciatus Microbiology Western blot Insect Science Bacillus thuringiensis parasitic diseases medicine |
| Zdroj: | Journal of Economic Entomology. 106:1098-1102 |
| ISSN: | 0022-0493 |
| Popis: | Bacillus thuringiensis (Bt) (Berliner) strain LLP29 produces a crystal protein Cyt1Aa6 toxic to mosquito vectors of human diseases. However, the susceptibility of Culex quinquefasciatus (Say) in the current study was 8.25 times higher than that of Aedes albopictus (Skuse) with this single protein Cyt1Aa6 purified from LLP29. To understand the mechanism of the novel mosquitocidal protein, the binding characteristic of brush border membrane vesicles from the two tested mosquitoes was investigated. Enzyme-linked immunosorbent assay showed that Cyt1Aa6 bound to the two mosquitoes’ brush border membrane vesicles. However, the titer of Ae. albopictus was a little higher than that of Cx. quinquefasciatus, with 3.21 and 2.91, respectively. Ligand Western blot analysis showed Cyt1Aa6 toxin specifically bound to the same three proteins (i.e., 68, 54, and 26 kDa) in the two mosquitoes, but one another protein, approximately to 37 kDa, could just be detected in Cx. quinquefasciatus. However, little differenc... |
| Databáze: | OpenAIRE |
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