Oxygen Insensitive Glucose Biosensor Based on PQQ-Dependent Glucose Dehydrogenase
| Autor: | Valdas Laurinavicius, Rolandas Rudomanskis, Rolandas Meškys, V. Liauksminas, Arunas Ramanavicius, Bogumila Kurtinaitiene, L. Boguslavsky, Terje Skotheim |
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| Rok vydání: | 1999 |
| Předmět: |
Chromatography
Immobilized enzyme Chemistry Biochemistry (medical) Clinical Biochemistry technology industry and agriculture Enzyme electrode Chemical modification macromolecular substances Biochemistry Reference electrode Analytical Chemistry Glucose dehydrogenase Electrochemistry Organic chemistry Fluorescent glucose biosensor Biosensor Spectroscopy Fumed silica |
| Zdroj: | Analytical Letters. 32:299-316 |
| ISSN: | 1532-236X 0003-2719 |
| DOI: | 10.1080/00032719908542822 |
| Popis: | Oxygen insensitive glucose biosensors based on PQQ-dependent glucose dehydrogenase from Erwinia sp. 34-1 and carbon paste have been designed. Without special precautions the sensitivity of the biosensors with the immobilized enzyme was low. Several methods of enzyme immobilization were compared. The enzyme was immobilized in the polylysine-albumin gel and in a paste consisting of water insoluble organic mediator, chemically modified carbon powder, fumed silica and binding material. Chemical modification of carbon with quinonic water insoluble mediators and application of fumed silica in the paste considerably improves the reproducibility, stability and sensitivity of the biosensors. The anodic response current of the biosensor on glucose was recorded at 0-200 mV vs Ag/AgCl reference electrode. Sensitivity can be increased more than 100 times in the presence of soluble mediators, such as phenazine methosulphate. Linearity of the biosensor can be extended by coating the enzymatic layer of the biose... |
| Databáze: | OpenAIRE |
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