Mechanism of TiO2nanoparticle-induced neurotoxicity in zebrafish (Danio rerio)
Autor: | Fashui Hong, Yunting Zhu, Ling Wang, Xiaohong Yu, Mingyu Su, Lei Sheng, Jie Hong, Xiaoyang Zhao, Renping Hu, Han Wang, Bingqing Xu, Dong Liu |
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Rok vydání: | 2014 |
Předmět: |
Neurotoxicity Syndrome
Health Toxicology and Mutagenesis Danio 010501 environmental sciences Management Monitoring Policy and Law Pharmacology Toxicology CREB 01 natural sciences 03 medical and health sciences 0302 clinical medicine Neurochemical medicine Zebrafish 0105 earth and related environmental sciences biology Neurotoxicity General Medicine Anatomy biology.organism_classification medicine.disease medicine.anatomical_structure Toxicity biology.protein Neuroglia 030217 neurology & neurosurgery |
Zdroj: | Environmental Toxicology. 31:163-175 |
ISSN: | 1520-4081 |
Popis: | Zebrafish (Danio rerio) has been used historically for evaluating the toxicity of environmental and aqueous toxicants, and there is an emerging literature reporting toxic effects of manufactured nanoparticles (NPs) in zebrafish embryos. Few researches, however, are focused on the neurotoxicity on adult zebrafish after subchronic exposure to TiO2 NPs. This study was designed to evaluate the morphological changes, alterations of neurochemical contents, and expressions of memory behavior-related genes in zebrafish brains caused by exposures to 5, 10, 20, and 40 μg/L TiO2 NPs for 45 consecutive days. Our data indicated that spatial recognition memory and levels of norepinephrine, dopamine, and 5-hydroxytryptamine were significantly decreased and NO levels were markedly elevated, and over proliferation of glial cells, neuron apoptosis, and TiO2 NP aggregation were observed after low dose exposures of TiO2 NPs. Furthermore, the low dose exposures of TiO2 NPs significantly activated expressions of C-fos, C-jun, and BDNF genes, and suppressed expressions of p38, NGF, CREB, NR1, NR2ab, and GluR2 genes. These findings imply that low dose exposures of TiO2 NPs may result in the brain damages in zebrafish, provide a developmental basis for evaluating the neurotoxicity of subchronic exposure, and raise the caution of aquatic application of TiO2 NPs. |
Databáze: | OpenAIRE |
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