| Autor: |
Gerd Jürgens, Sunethra Dharmasiri, Nihal Dharmasiri, Dolf Weijers, Nirmala Karunarathna, Mark Estelle |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
The Plant Journal. 52:114-123 |
| ISSN: |
0960-7412 |
| DOI: |
10.1111/j.1365-313x.2007.03211.x |
| Popis: |
Cullin-RING ubiquitin-protein ligases such as the Skp1, cullin, F-box protein (SCF) have been implicated in many growth and developmental processes in plants. Normal SCF function requires that the CUL1 subunit be post-translationally modified by related to ubiquitin (RUB), a protein related to ubiquitin. This process is mediated by two enzymes: the RUB-activating and RUB-conjugating enzymes. In Arabidopsis, the RUB-activating enzyme is a heterodimer consisting of AXR1 and ECR1. Mutations in the AXR1 gene result in a pleiotropic phenotype that includes resistance to the plant hormone auxin. Here we report that the AXL (AXR1-like) gene also functions in the RUB conjugation pathway. Overexpression of AXL in the axr1-3 background complements the axr1-3 phenotype. Biochemical analysis indicates that AXL overexpression restores CUL1 modification to the wild-type level, indicating that AXR1 and AXL have the same biochemical activity. Although the axl mutant resembles wild-type plants, the majority of axr1 axl-1 double mutants are embryo or seedling lethal. Furthermore, the axl-1 mutation reveals novel RUB-dependent processes in embryo development. We conclude that AXR1 and AXL function redundantly in the RUB conjugating pathway. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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