Preparation of low-molecular-weight citrus pectin by recombinant Bacillus subtilis pectate lyase and promotion of growth of Bifidobacterium longum
Autor: | Xian-jun Dai, Ya-hui Dang, Ming-qi Liu, Wen-kang Huo |
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Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine Bifidobacterium longum Bacillus subtilis medicine.disease_cause 01 natural sciences Catalysis law.invention 03 medical and health sciences Hydrolysis law 010608 biotechnology medicine Citrus Pectin Food science Escherichia coli Molecular mass biology Chemistry Process Chemistry and Technology food and beverages General Chemistry biology.organism_classification 030104 developmental biology Pectate lyase Recombinant DNA bacteria |
Zdroj: | Catalysis Communications. 107:39-42 |
ISSN: | 1566-7367 |
DOI: | 10.1016/j.catcom.2018.01.017 |
Popis: | pel gene (GenBank: KM244046.1 ), which encodes Bacillus subtilis pectate lyase (PelB), was high-level expressed in Escherichia coli BL21 (DE3) and the recombinant enzyme was named rePelB. After induction by IPTG at 15 °C for 24 h, specific activity of rePelB in culture supernatant of pCpel-6 reached 197.4 ± 5.20 U/mg. The optima of rePelB were 60 °C and pH 5.0, respectively. Hydrolysis of citrus pectin by rePelB in 50 L grass reactor was optimized by implementing RSM. HPLC analysis revealed molecular mass of citrus pectin and obtained LCP was 290 and 5.8 kDa, respectively. LCP can significantly promote growth of Bifidobacterium longum. |
Databáze: | OpenAIRE |
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