Cellular Mechanisms and Modulation of Activin A- and Transforming Growth Factor β-Mediated Differentiation in Cultured Hen Granulosa Cells1
| Autor: | Dori C. Woods, Alan L. Johnson, Jamie T. Bridgham |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Granulosa cell differentiation
endocrine system medicine.medical_specialty Cholesterol side-chain cleavage enzyme luteinizing hormone/choriogonadotropin receptor Cell Biology General Medicine Biology Cell biology medicine.anatomical_structure Endocrinology Reproductive Medicine Internal medicine TGF beta signaling pathway medicine Ovarian follicle Follicle-stimulating hormone receptor hormones hormone substitutes and hormone antagonists Activin type 2 receptors Transforming growth factor |
| Zdroj: | Biology of Reproduction. 71:1844-1851 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.104.032573 |
| Popis: | Undifferentiated granulosa cells from prehierarchal (6- to 8-mm-diameter) hen follicles express very low to undetectable levels of LH receptor (LH-R) mRNA, P450 cholesterol side chain cleavage (P450scc) enzyme activity, and steroidogenic acute regulatory (StAR) protein, and produce negligible progesterone, in vitro, following an acute (3-h) challenge with either FSH or LH. It has previously been established that culturing such cells with FSH for 18–20 h induces LH-R, P450scc, and StAR expression, which enables the initiation of progesterone production. The present studies were conducted to characterize the ability of activin and transforming growth factor (TGF) β, both alone and in combination with FSH, to promote hen granulosa cell differentiation, in vitro. A 20-h culture of prehierarchal follicle granulosa cells with activin A or transforming growth factor β (TGFβ)1 increased LH-R mRNA levels compared with control cultured cells. Activin A and TGFβ1 also promoted FSH-receptor (FSH-R) mRNA expr... |
| Databáze: | OpenAIRE |
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