Autor: Pospelova Tv, Nikolai D. Aksenov, Valery A. Pospelov, A. I. Brichkina, Bulavin Dv, Tararova Nd
Rok vydání: 2002
Předmět:
Zdroj: Molecular Biology. 36:44-49
ISSN: 0026-8933
DOI: 10.1023/a:1014242322335
Popis: Introduction of the E1A early region of the human adenovirus type 5 impairs the ability of mammalian cells to stop in the cell cycle at G1/S after damage. Two-parameter fluorescence cell sorting with iododeoxyuridine revealed the radiation-induced G1/S arrest in rat embryo fibroblasts transformed with the complementing E1A and E1B-19kDa oncogenes. This was due to selective inhibition of CyclE/Cdk2-associated kinase activity, while activities of type 2 kinase and of CyclA/Cdk2 complexes remained unchanged. The inhibitor of G1-phase cyclin kinases, p21/Waf1, was accumulated and interacted with target kinases both in normal and in transformed cells after irradiation. As shown by immunoprecipitation, p21/Waf1 formed complexes with the E1A oncoproducts in the transformants, which possibly accounted for its functional inactivation. Kinase modification in cyclin–kinase complexes was assumed to play a key role in regulation of cyclin-dependent kinases in the transformants with inactivated p21/Waf1.
Databáze: OpenAIRE
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