Determination of Zinc in Serum, Blood, and Ultrafiltrate Fluid from Patients on Hemofiltration by Graphite Furnace/Atomic Absorption Spectroscopy or Flow Injection Analysis/Atomic Absorption Spectroscopy
| Autor: | S V Gonzalez, J A Tomero, O.J. de Blas, Jesús Hernández Méndez, R S Rodriguez, B de L Gomez |
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| Rok vydání: | 1994 |
| Předmět: |
Pharmacology
Flow injection analysis Detection limit Chromatography medicine.medical_treatment Analytical chemistry chemistry.chemical_element Venous blood Zinc Analytical Chemistry law.invention chemistry law Hemofiltration medicine Environmental Chemistry Arterial blood Atomic absorption spectroscopy Graphite furnace atomic absorption Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 77:722-727 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/77.3.722 |
| Popis: | Two methods were optimized for the determination of zinc in samples of blood, serum, and ultrafiltrate fluid from patients with chronic renal impairment undergoing hemofiltration. In the first procedure, after acid digestion of the samples, Zn in blood and serum is determined by a system coupled to flow injection analysis and atomic absorption spectros-copy. The method is rapid, automated, simple, needs small amounts of sample, and has acceptable analytical characteristics. The analytical characteristics obtained were as follows: determination range of method, 0.05-2.0 ppm of Zn; precision as coefficient of variation (CV), 5.3%; recovery, 95-105%; and detection limit (DL), 0.02 ppm. The second method is optimized for ultrafiltrate fluid because the sensitivity of the first procedure is not suitable for the levels of Zn (ppb or ng/mL) in these samples. The technique chosen was atomic absorption spectroscopy with electrothermal atomization in a graphite furnace. The analytical characteristics obtained were as follows: determination range of method, 0.3-2.0 ppb Zn; CV, 5.7%; recovery, 93-107%; and DL, 0.12 ppb. The methods were used to determine zinc in samples of blood, serum, and ultrafiltrate fluid from 5 patients with chronic renal impairment undergoing hemofiltration to discover whether there were significant differences in the zinc contents of blood, serum, and ultrafiltrate fluid after the hemofiltration process. An analysis of variance of the experimental data obtained from a randomly selected group of 5 patients showed that zinc concentrations in the ultrafiltrate fluid, venous blood, and venous serum do not vary during hemofiltration (p < 0.05), whereas in arterial blood and serum, the time factor has a significant effect. |
| Databáze: | OpenAIRE |
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