Apocynin Exerts Dose-Dependent Cardioprotective Effects by Attenuating Reactive Oxygen Species in Ischemia/Reperfusion: A Recent Study
| Autor: | Harsh Patel, Lindon H. Young, Issachar Devine, Robert Barsotti, Tarah Kuhn, Kyle D. Bartol, Tsion Habtamu, C. Woodworth Parker, Regina Ondrasik, Brendan Casey, William Chau, Qian Chen |
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| Rok vydání: | 2021 |
| Předmět: |
Cardiac function curve
chemistry.chemical_classification medicine.medical_specialty Reactive oxygen species NADPH oxidase biology Ischemia medicine.disease Nitric oxide chemistry.chemical_compound Endocrinology chemistry Internal medicine Apocynin cardiovascular system medicine biology.protein cardiovascular diseases Endothelial dysfunction Reperfusion injury |
| DOI: | 10.9734/bpi/nfmmr/v7/8739d |
| Popis: | Ischemia/reperfusion results in cardiac contractile dysfunction and cell death partly due to increased reactive oxygen species and decreased endothelial-derived nitric oxide bioavailability. Ischemia/reperfusion injury is initiated in part by endothelial dysfunction, which occurs within 5 min of reperfusion. NADPH oxidase normally produces reactive oxygen species to facilitate cell signalling and differentiation; however, excessive release of such species following ischemia exacerbates cell death. Thus, administration of an NADPH oxidase inhibitor, apocynin, may preserve cardiac function and reduce infarct size following ischemia. Apocynin dose-dependently (40 \(\mu\)M, 400 \(\mu\)M and 1 mM) attenuated leukocyte superoxide release by 87 ± 7%. Apocynin was also given to isolated perfused hearts after ischemia, with infarct size decreasing to 39 ± 7% (40 \(\mu\)M), 28 ± 4% (400 \(\mu\)M; p < 0.01) and 29 ± 6% (1 mM; p < 0.01), versus the control’s 46 ± 2%. This decrease correlated with improved final post-reperfusion left ventricular end-diastolic pressure, which decreased from 60 ± 5% in control hearts to 56 ± 5% (40 \(\mu\)M), 43 ± 4% (400 \(\mu\)M; p < 0.01) and 48 ± 5% (1 mM; p < 0.05), compared to baseline. Functionally, apocynin (13.7 mg/kg, I.V.) significantly reduced H2O2 by nearly four-fold and increased endothelial-derived nitric oxide bioavailability by nearly four-fold during reperfusion compared to controls (p < 0.01), which was confirmed in in vivo rat hind limb ischemia/reperfusion models. These results suggest that apocynin attenuates ischemia/reperfusion-induced cardiac contractile dysfunction and infarct size by inhibiting reactive oxygen species release from NADPH oxidase. |
| Databáze: | OpenAIRE |
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